Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

LncRNA gas5 regulates granulosa cell apoptosis and viability following radiation by x-ray via sponging miR-205-5p and Wnt/?-catenin signaling pathway in granulosa cell tumor of ovary

Yan Li¹, Xing Ma², Jun Li¹, Saifei He², Juhua Zhuang², Guoyu Wang², Ying Ye², Wei Xia²

¹Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine; ²Department of Nuclear Medicine, The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China.

For correspondence:- Wei Xia Email: weixia1911@163.com Tel:+8613277726355

Accepted: 21 Mar 2020 Published: 30 June 2020

Citation: Li Y, Ma X, Li J, He S, Zhuang J, Wang G, et al. LncRNA gas5 regulates granulosa cell apoptosis and viability following radiation by x-ray via sponging miR-205-5p and Wnt/?-catenin signaling pathway in granulosa cell tumor of ovary. Trop J Pharm Res 2020; 19(6):1153-1159 doi: 10.4314/tjpr.v19i6.5

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the role of lncRNA gas5 in ovarian granulosa cells exposed to x-ray in granulosa cell tumor of ovary (GCTO).

Methods: KGN cell line was exposed to X-ray to mimic the radiotherapy for GCSO patients in vitro, cell viability was checked by CCK8 assays. RNA expression of apoptosis-related genes was determined by quantitative reverse transcriptase-polymerase reaction (qRT-PCR) while Western Blot for biomarkers in wnt/β-catenin signaling. Differential expressions of lncRNA gas5 were examined after cells were exposed to a ray for 0,24,48hs. We over expressed gas5 and assessed resultant cell viability, apoptosis and signaling. The sponging between gas5 and miR-205-5p was verified by luciferase assay. CCK8, qRT-PCR and Western blot were applied to investigate the correlation between miR-205-5p, cell viability, and apoptosis after miR-205-5p augmentation. Similarly, interaction between gas5 and miR-205-5p was assessed after co-transfection of miR-205-5p mimics and oe-gas5. Finally, wnt inhibitor was used to study the role of signaling pathway in KGN cells.

Results: Exposure of KGN to x-ray reduced cell viability and increased apoptosis. Gas5showed reduced expression in the cells, while miR-205-5p expression increased. Gas5 upregulation protected the cells against apoptosis and contributed to cell viability and activation of wnt//β-catenin signaling. lncRNA gas5 targeted miR-205-5p and miR-205-5p mimics counteracted the functions of up-regulated lncRNA gas5, regulating Wnt/β-catenin signaling pathway. Inactivation of Wnt/β-catenin suppressed cell viability.

Conclusions: lncRNA gas5 regulates cell apoptosis and viability following cellular radiation, thus presenting a potential therapeutic target for the application radiotherapy in GCTO patients.

Keywords: Ovary, Proliferation, Apoptosis, lncRNA gas5, Radiotherapy, ?-catenin signaling